Extracellular vesicles: We discovered that most ciliated neurons release extracellular vesicles (EVs) from their cilia ending by outward budding of cilia plasma membrane. Ciliary EV release contributes to maintain cilia composition and sensory function. Potentially they may carry bioactive material between worms. How does EV biogenesis and release occur and what are their biological function ?
Neurosecretion: We explore the cell biology of Synaptic Vesicle (SV) and Dense Core Vesicle (DCV) using forward genetics and candidate gene approaches. We identified conserved pathways involved in DCV biogenesis, traffic and secretion in neurons. We showed neuropeptides contribute to cross-talks between neuron within and between circuits. How neuropeptide release, DCV biogenesis and traffic are modulated in the nervous system?
Neuromuscular ageing: We study neuromuscular ageing through the progression in worm locomotion. Combining multiples features of worms’ locomotion we can predict the age of the individuals. How such a stereotypical behavioural progression is generated by the nervous system?
Neuronal atlas: We generated a molecular atlas of 62 neuron classes of C. elegans at the second larval stage and provided many new cell-specific promoters. We collaborated to identify sexual dimorphisms within sex-shared neurons. How molecular dimorphisms generate sex-specific behaviour?
Oxygen sensing: Atmospheric O2 is aversive for C. elegans. During My post doc, we identified neuroglobin and guanylate cyclases, as necessary sensors for high O2. The O2 sensory neurons are tonic sensors showing little or no adaptation to the cues. Via the release of neuropeptides, they regulate behaviour and metabolism.
